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The recently reported structure of the HCE–LD4AC complex displayed an unexpected binding mode, in which HCE binds to the side of the β-helical bundle of LD4 , Supplementary Fig. 12f,. In addition (Arg1064 in BoNT/A2 is replaced by His1064 in BoNT/A1), which plays a similar role in the interaction with NAG attached to LD4C.
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